Teoretical and practical aspects of animal cell cultures

This course addresses to the students from Master’s cycle (1st year of study) – Master of Biochemistry and Molecular Biology. Specific topics include:

1. History, objectives, advantages and disadvantages of animal cell cultures.

2. Cell culture systems: tissue, organ and cell cultures; organotypic and histotypic cultures; mass and clonal cell cultures; adherent cell culture vs. suspension cell culture; primary cell cultures, finite cell lines and continuous cell lines.

3. Cell culture media and cell culture conditions

4. Growth cycle and cell proliferation in culture.

5. Cell syncronization: chemical and physical methods.

6. Cell immortalization: Spontaneous immortalization; Methods to obtain immortal cells (DNA tumor viruses, telomere maintainance, ALT-Alternative Lenghtening of Telomeres, expression of adenoviral and retroviral oncogenes, carcinogens and irradiation); Practical aspects of the immortalization of primary cells.

7. Cell transfection: Types of transfection; Cell culture conditions to transfect cells in culture; Methods for cell transfection; Reporter gene systems.

8. Cell transformation: Genomic instability; Cell immortality; Aberrant cell growth control; Tumorigenicity of transformed cells.

9. Cellular senescence: Types of cellular senescence and characteristics of senescent cells; The signals and molecular pathways activating the cellular senescence; Mechanisms of induction of cellular senescence.

10. Cell quiescence, cell differentiation  and cell de-differentiation.

11. Flow cytometry;

12.  Application of cell cultures in obtaining monoclonal antibodies.

 

 

 

 

 

 

 

Last updated at: June 21, 2016.