Proteomics

 

Course description 

The Proteomics course is designed for the IInd year students of the following Master Programmes: Biochemistry and Molecular Biology (in Romanian), Applied Biochemistry and Molecular Biology (in English) and Applied Genetics and Biotechnologies (in Romanian), Faculty of Biology, University of Bucharest.

The course addresses the following topics:

(i) Introduction in Proteomics (amino acids, peptides, proteins, proteome, protein extraction);

(ii) Separation of proteins (ultracentrifugation, precipitation, dialysis, chromatography, electrophoresis);

(iii) Quantification of proteins;

(iv) Identification of proteins (complete hydrolysis, Edman degradation, mass spectrometry, protein sequence analysis, immunological techniques);

(v) Interaction Proteomics (biochemical, genetic, bioinformatic, optical methods);

(vi) Structural Proteomics (X-Ray Crystallography, NMR, 3D Cryo EM, molecular modeling);

(vii) Modifications of proteins (phosphorylation, glycosylation, etc.);

(viii) Applications of Proteomics (disease diagnosis, drug design, plant biotechnologies). 

 

References

Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P. Molecular Biology of the Cell (5th Edition). Taylor & Francis Ltd., 2007. ISBN: 978-0815341055.

Berg JM, Tymoczko JL, Stryer L. Biochemistry (7th Edition). WH Freeman, 2011. ISBN: 978-1429276351.

Dinischiotu A, Costache M. Biochimie Generală (vol. I) - Proteine, Glucide, Lipide. Ars Docendi, 2004. ISBN: 973-5581337.

Iordăchescu D. Biochimia Aminoacizilor și Proteinelor. University of Bucharest, 1995.

Nelson DL, Cox MM. Lehninger Principles of Biochemistry (6th Edition). WH Freeman, 2013. ISBN: 978-1464109621.

Rehm H. Protein Biochemistry and Proteomics (4th Edition). Elsevier Inc., 2006. ISBN: 978-0120885459.

Rhodes G. Crystallography Made Crystal Clear (3rd Edition). Elsevier / Academic Press, 2006. ISBN: 978-0125870733.

Rosenberg IM. Protein analysis and purification: benchtop techniques (2nd Edition). Birkhäuser, 2004. ISBN: 978-0817643416.

Twyman RM. Principles of Proteomics. BIOS Scientific Publishers, 2004. ISBN: 978-1859962732.

Zăulet M, Costache M. Lucrări Practice de Biochimie și Biologie Moleculară (vol. I). University of Bucharest, 2011. 

 

More information concerning both "Biochemistry and Molecular Biology" and "Applied Biochemistry and Molecular Biology" Master Programmes:

http://www.bio.unibuc.ro/index.php?option=com_content&view=article&id=98&Itemid=86

 

 

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Installed at 23.07.14

 

 

 

Last updated at: January 23, 2017.